CAPILLARY ZONE ELECTROPHORESIS SEPARATION AND LASER-BASED DETECTION OF BOTH FLUORESCEIN THIOHYDANTOIN AND DIMETHYLAMINOAZOBENZENE THIOHYDANTOIN DERIVATIVES OF AMINO-ACIDS

被引：30

作者：

WALDRON, KC ^{[1
]}

WU, SL ^{[1
]}

EARLE, CW ^{[1
]}

HARKE, HR ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 09期

关键词：

D O I：

10.1002/elps.1150110918

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary zone electrophoresis is employed for the separation and analysis of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids. Detection of minute amounts of these amino acid derivatives is an important milestone in the development of a high sensitivity protein sequencer. Current detection limits for the fluorescein derivative is on the order of 10−21 moles whereas detection limits for the dimethylaminoazobenzene derivative is on the order of 10−16 moles. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：777 / 780

页数：4

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