ISOLATION, SEQUENCE-ANALYSIS AND CHARACTERIZATION OF A CDNA-ENCODING HUMAN CHAPERONIN-10

被引:6
|
作者
CHEN, JJ
MCNEALY, DJ
DALAL, S
ANDROPHY, EJ
机构
[1] TUFTS UNIV NEW ENGLAND MED CTR,DEPT DERMATOL,BOSTON,MA 02111
[2] TUFTS UNIV,SCH MED,DEPT MOLEC BIOL & MICROBIOL,BOSTON,MA 02111
关键词
CLONING; CHAPERONIN; NUCLEOTIDE SEQUENCE; (HUMAN);
D O I
10.1016/0167-4781(94)90268-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A full-length cDNA clone encoding chaperonin 10 (cpn10) from a HeLa cell cDNA library was isolated: The cDNA is 538 bp in length, contains an ATG codon and a putative polyadenylation signal, and specifies a protein of 102 amino acids. Immunoprecipitation experiment showed that this human cpn10 has an apparent molecular mass of 11 kDa in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
引用
收藏
页码:189 / 190
页数:2
相关论文
共 50 条