APPLICATION OF STATISTICAL DESIGN TO THE OPTIMIZATION OF CULTURE MEDIUM FOR BIOMASS PRODUCTION BY EXIGUOBACTERIUM SP. HM 119395

被引：0

作者：

Kiruthika, Panneerselvam ^{[1
]}

Nisshanthini, S. Durairaju ^{[2
]}

Saraswathi, Ayyaswamy ^{[2
]}

Angayarkanni, Jayaraman ^{[2
]}

Rajendiran, Rasappan ^{[3
]}

机构：

[1] Bharathiar Univ, Res & Dev Ctr, Coimbatore 641046, Tamil Nadu, India

[2] Bharathiar Univ, Dept Microbial Biotechnol, Canc Therapeut Lab, Coimbatore 641046, Tamil Nadu, India

[3] Poultech Agrores Ctr, Namakkal, India

来源：

INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH | 2011年 / 2卷 / 04期

关键词：

Exiguobacterium sp; Media optimization; Growth; Plackett-Burman design and Central composite design;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The central composite design was developed to search for an optimal medium for the growth of Exiguobacterium sp. The effect of various media components, such as sucrose, peptone, yeast extract, sea water, glycerol, pH, incubation periods and inoculum size were examined. The first-order model based on Plackett-Burman design showed that sea water, pH and sucrose and glycerol influenced the growth of the examined bacteria. The second-order polynomial regression confirmed that maximum biomass production was achieved by the combination of pH (8.7), sea water (350 ml), sucrose (2.0 g) and glycerol (0.5 ml) with the predicted maximum biomass production of 0.8116 O. D of fermented media. The organism produced 0.807 O.D. confirming the validity.

引用

页码：422 / 430

页数：9

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