APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA USING THE POLYMERASE CHAIN-REACTION FOR EFFICIENT ELIMINATION OF DUPLICATE STRAINS IN MICROBIAL SCREENING .3. BACTERIA

被引：6
|
作者：
TANAKA, H [1 ]
SAWAIRI, S [1 ]
OKUDA, T [1 ]
机构：
[1] NIPPON ROCHE RES CTR,DEPT MICROBIOL & TAXON,KAMAKURA,KANAGAWA 247,JAPAN
来源：
JOURNAL OF ANTIBIOTICS | 1994年 / 47卷 / 02期
关键词：
D O I：
10.7164/antibiotics.47.194
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Random amplified polymorphic DNA (RAPD) analysis was evaluated for the selection and elimination of bacterial strains used in microbial screening. For this pilot study we used eight bacterial strains producing fragin and two Pseudomonas fragi strains, which are often isolated during the screening. A dendrogram constructed by the statistical analysis using parsimony, PAUP, based on the band patterns of RAPD with primer R28 was in good correlation with the results of DNA - DNA hybridization, HPLC analysis of metabolites, and conventional morphological and physiological characterization. RAPD was also applicable to a wide range of bacteria. This rapid selection system by RAPD was a very useful tool for excluding similar bacterial isolates encountered during screening.
引用
收藏
页码：194 / 200
页数：7
相关论文
共 50 条
  • [31] Application of polymerase chain reaction (PCR) using random amplified polymorphic DNA (RAPD) primers in the molecular identification of selected Sargassum species (Phaeophyta, Fucales)
    Ho, CL
    Phang, SM
    Pang, T
    EUROPEAN JOURNAL OF PHYCOLOGY, 1995, 30 (04) : 273 - 280
  • [32] VALIDATION OF A METHOD FOR THE PREPARATION OF FUNGAL GENOMIC DNA FOR POLYMERASE CHAIN-REACTION (PCR) AND RANDOM AMPLIFICATION OF POLYMORPHIC DNA (RAPD)
    VANVAERENBERGH, B
    GROOTAERT, B
    MOENS, W
    JOURNAL DE MYCOLOGIE MEDICALE, 1995, 5 (03): : 133 - 139
  • [33] Random amplified polymorphic DNA and polymerase chain reaction markers for the differentiation and detection of Stenocarpella maydis in maize seeds
    Xia, Z
    Achar, PN
    JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT, 2001, 149 (01): : 35 - 44
  • [34] A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species
    Rivas, R
    Velázquez, E
    Valverde, A
    Mateos, PF
    Martínez-Molina, E
    ELECTROPHORESIS, 2001, 22 (06) : 1086 - 1089
  • [35] A specific primer pair for the diagnosis and identification of Acanthamoeba astronyxis by random amplified polymorphic DNA polymerase chain reaction
    Ortega-Rivas, A
    Lorenzo-Morales, J
    Martínez, E
    Villa, M
    Clavel, A
    Valladares, B
    del Castillo, A
    JOURNAL OF PARASITOLOGY, 2005, 91 (01) : 122 - 126
  • [36] A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species
    Ral Rivas, Encarna Velzquez
    Valverde, Angel
    Mateos, Pedro F.
    Martnez-Molina, Eustoquio
    2001, Wiley-VCH Verlag (22)
  • [37] AMPLIFICATION OF DNA FROM NATIVE POPULATIONS OF SOIL BACTERIA BY USING THE POLYMERASE CHAIN-REACTION
    BRUCE, KD
    HIORNS, WD
    HOBMAN, JL
    OSBORN, AM
    STRIKE, P
    RITCHIE, DA
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (10) : 3413 - 3416
  • [38] DIRECT COMPLEMENTARY-DNA CLONING AND SCREENING OF MUTANTS USING POLYMERASE CHAIN-REACTION
    CHIANG, YL
    METHODS IN ENZYMOLOGY, 1992, 216 : 73 - 80
  • [39] EFFICIENT COMPLEMENTARY-DNA AMPLIFICATION AND EXPRESSION USING POLYMERASE CHAIN-REACTION TECHNOLOGY
    PAULS, TL
    BERCHTOLD, MW
    METHODS IN ENZYMOLOGY, 1993, 217 : 102 - 122
  • [40] Assessment of Arsenic-Induced DNA Damage in Goldfish by a Polymerase Chain Reaction-Based Technique Using Random Amplified Polymorphic DNA Markers
    Kumar, Amod
    Kesari, Vibudh P.
    Alok, Ashok Kumar
    Kazim, Syed N.
    Khan, Parimal K.
    ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2014, 67 (04) : 630 - 638
12345