APICAL MEMBRANE LOCALIZATION OF OUABAIN-SENSITIVE K+-ACTIVATED ATPASE ACTIVITIES IN RAT DISTAL COLON

被引:96
|
作者
DELCASTILLO, JR
RAJENDRAN, VM
BINDER, HJ
机构
[1] YALE UNIV, SCH MED, DEPT INTERNAL MED, 333 CEDAR ST, NEW HAVEN, CT 06510 USA
[2] INST VENEZOLANO INVEST CIENT, CTR BIOFIS & BIOQUIM, CARACAS 1020A, VENEZUELA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 06期
关键词
OUABAIN-INSENSITIVE POTASSIUM-ACTIVATED ADENOSINE-TRIPHOSPHATASE;
D O I
10.1152/ajpgi.1991.261.6.G1005
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
This study sought to establish the presence of K+-activated adenosinetriphosphatase (ATPase) activity in the colonic mucosa of the rat distal colon. K+-activated ATPase activity was present in apical membranes but not in basolateral membranes. K+-activated ATPase activity in apical membranes represented an approximate 10-fold enrichment compared with that in the homogenate. Na+-K+-activated ATPase activity was also present in homogenate but was enriched less than fourfold in apical membranes. K+-activated ATPase activity in apical membranes had both ouabain-sensitive and ouabain-insensitive components. In contrast, Na+-K+-activated ATPase activity was completely inhibited by ouabain. Similar half-maximal concentrations for K+ and pH activation curves were found for both ouabain-sensitive and ouabain-insensitive fractions. In addition to K+, the ouabain-sensitive fraction of K+-activated ATPase activity was stimulated by Rb+, NH4+, and Cs+, whereas the ouabain-insensitive fraction was activated only by Rb+. K+-activated ATPase activity was significantly inhibited by vanadate but not by N-ethylmaleimide or omeprazole. In the proximal colon, in contrast to the distal colon, active K+ absorption is not present, and K+-activated ATPase is approximately 20% of that in the distal colon. These studies demonstrate that K+-activated ATPase is present in apical membranes of rat distal colon and permit the speculation that this enzyme represent a unique and distinct ATPase (compared with either Na+-K+-ATPase or gastric parietal cell K+-ATPase) and is likely linked closely to the active K+ absorptive process present in this epithelium.
引用
收藏
页码:G1005 / G1011
页数:7
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