EVALUATION OF A TECHNIQUE FOR SAMPLING BLOOD FROM SMALL FISHES

被引：2
|
作者：
ALLEN, P
机构：
[1] Department of Zoology, National University of Singapore, Singapore
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 1994年 / 107卷 / 02期
关键词：
HEMATOLOGICAL PARAMETERS; BLOOD; SMALL FISHES; OREOCHROMIS AUREUS; SAMPLING BLOOD;
D O I：
10.1016/0300-9629(94)90400-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A technique has been developed for extracting small quantities of blood, up to 16 mu l, from fish of 20-30 mm total length, from the dorsal aorta of decapitated fish. This method can provide up to 4 mu l of blood from fish of total length 10 mm. This method compares favourably with the well established technique of caudal peduncle severance in larger fish; no significant difference was found between the two techniques for plasma osmolality, HGBC, WBCC and HCT.
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页码：413 / 418
页数：6
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