PURIFICATION OF A BASIC PHOSPHOLIPASE A(2) FROM INDIAN SAW-SCALED VIPER (ECHIS-CARINATUS) VENOM - CHARACTERIZATION OF ANTIGENIC, CATALYTIC AND PHARMACOLOGICAL PROPERTIES

被引：22
|
作者：
KEMPARAJU, K [1 ]
PRASAD, BN [1 ]
GOWDA, VT [1 ]
机构：
[1] UNIV MYSORE,DEPT STUDIES BIOCHEM,MYSORE 570006,KARNATAKA,INDIA
来源：
TOXICON | 1994年 / 32卷 / 10期
关键词：
D O I：
10.1016/0041-0101(94)90348-4
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A major basic phospholipase A(2) was purified from the Indian saw-scaled viper (Echis carinatus) venom by the combination of column chromatography and electrophoresis. The purified phospholipase A(2) (EC-IV-PLA(2)) has a mel. wt of 14,000 by SDS-PAGE. It is a basic protein with a pI value between 7.2 and 7.6, and has a fluorescence emission maxima at 340 nm. It induces neurotoxicity and oedema in mice with an i.p. LD(50) Of 5 mg/kg body weight. It is devoid of direct haemolytic, myotoxic, cytotoxic and anticoagulant activities. Rabbit polyclonal antibodies prepared against EC-IV-PLA(2) inhibited the in vitro enzymatic activity dose dependently, but did not neutralize the toxic effects of EC-IV-PLA(2) in experimental animals.
引用
收藏
页码：1187 / 1196
页数：10
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