IDENTIFICATION OF AN INTRACELLULAR PYRIMIDINE-SPECIFIC ENDORIBONUCLEASE FROM BACILLUS-SUBTILIS

被引：8

作者：

MATHUR, S ^{[1
]}

CANNISTRARO, VJ ^{[1
]}

KENNELL, D ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,BOX 8230,ST LOUIS,MO 63110

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 20期

关键词：

D O I：

10.1128/jb.175.20.6717-6720.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two intracellular RNases which were easily separated by fractionation on strong anion- or cation-exchange resins were identified from Bacillus subtilis. One cleaved any phosphodiester bond, while the second cleaved only pyrimidine-N bonds. The enzyme with pyrimidine-N specificity was approximately 15 kDa, had a pH optimum of approximately 6.2, degraded C-C bonds approximately 10 times faster than U-U bonds, and was completely inactive against single-stranded DNA. The enzyme is called RNase C and may be the first reported broad-specificity endoribonuclease from B. subtilis.

引用

页码：6717 / 6720

页数：4

共 50 条

[41] IDENTIFICATION OF THE PLEIOTROPIC SACQ GENE OF BACILLUS-SUBTILIS
YANG, M
FERRARI, E
CHEN, E
HENNER, DJ
JOURNAL OF BACTERIOLOGY, 1986, 166 (01) : 113 - 119
[42] INTRACELLULAR SERINE PROTEINASE OF BACILLUS-SUBTILIS STRAIN MARBURG-168 - COMPARISON WITH THE HOMOLOGOUS ENZYME FROM BACILLUS-SUBTILIS STRAIN A-50
STRONGIN, AY
GORODETSKY, DI
KUZNETSOVA, IA
YANONIS, VV
ABRAMOV, ZT
BELYANOVA, LP
BARATOVA, LA
STEPANOV, VM
BIOCHEMICAL JOURNAL, 1979, 179 (02) : 333 - 339
[43] IDENTIFICATION OF THE PROMOTER OF THE BACILLUS-SUBTILIS SDH OPERON
MELIN, L
MAGNUSSON, K
RUTBERG, L
JOURNAL OF BACTERIOLOGY, 1987, 169 (07) : 3232 - 3236
[44] PURIFICATION AND PROPERTIES OF PHYTATE-SPECIFIC PHOSPHATASE FROM BACILLUS-SUBTILIS
POWAR, VK
JAGANNATHAN, V
JOURNAL OF BACTERIOLOGY, 1982, 151 (03) : 1102 - 1108
[45] IDENTIFICATION AND PURIFICATION OF A CALCIUM-BINDING PROTEIN FROM BACILLUS-SUBTILIS
TOZZI, MG
DARCANGELO, U
DELCORSO, A
ORDAL, GW
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1080 (02) : 160 - 164
[46] THE ISOLATION, CLONING AND IDENTIFICATION OF A VEGETATIVE CATALASE GENE FROM BACILLUS-SUBTILIS
BOL, DK
YASBIN, RE
GENE, 1991, 109 (01) : 31 - 37
[47] MAPPING OF GENES CONTROLLING EXCISION REPAIR OF PYRIMIDINE PHOTOPRODUCTS IN BACILLUS-SUBTILIS
MUNAKATA, N
MOLECULAR & GENERAL GENETICS, 1977, 156 (01): : 49 - 54
[48] STRUCTURE OF THE BACILLUS-SUBTILIS PYRIMIDINE BIOSYNTHETIC (PYR) GENE-CLUSTER
LERNER, CG
STEPHENSON, BT
SWITZER, RL
JOURNAL OF BACTERIOLOGY, 1987, 169 (05) : 2202 - 2206
[49] EFFECT OF PURINE AND PYRIMIDINE LIMITATIONS ON RNA-SYNTHESIS IN BACILLUS-SUBTILIS
VASANTHA, N
GALLIERS, EM
HANSEN, JN
JOURNAL OF BACTERIOLOGY, 1984, 158 (03) : 884 - 889
[50] FLAVOKINASE AND FAD SYNTHETASE FROM BACILLUS-SUBTILIS SPECIFIC FOR REDUCED FLAVINS
KEARNEY, EB
GOLDENBERG, J
LIPSICK, J
PERL, M
JOURNAL OF BIOLOGICAL CHEMISTRY, 1979, 254 (19) : 9551 - 9557

← 1 2 3 4 5 →