DESGINING, METHOD DEVELOPMENT AND STANDARDIZATION OF PROCESS FOR EXTRACTION OF MARKER COMPOUND FROM TERMINALIA CHEBULA FRUIT

The present study is done to study and optimize the process of enzyme-assisted extraction of Chebulinic acid from the dried fruit of Terminalia chebula. A selective extraction process after the treatment with enzymes is proposed by using 30% (v/v) methanol which releases increased yield of the Chebulinic acid, present in the dried fruit compare to aqueous extract. The optimal conditions were as follows: pH value was 4.5, concentration of cellulase solution was 2.5 mg/mL, incubation time was 8 h, and incubation temperature was 50 degrees C and solid: solvent ratio was 1:8. Enzymes have the ability to degrade or disrupt cell walls and membranes, thus enabling better release and more efficient extraction of bioactives. Enzyme-assisted extraction methods are gaining more attention because of the need for eco-friendly extraction technologies. Enzyme-assisted extraction was proven to be highly efficient in the designing, process development and standardization of extraction of marker compound from herbal drugs and could be used in making enriched phytopharmaceuticals which may be translated into herbal drug for treating a medical condition.