GENETIC-RELATIONSHIPS AMONG STRAINS OF XYLELLA-FASTIDIOSA FROM RAPD-PCR DATA

被引：68
|
作者：
POOLER, MR [1 ]
HARTUNG, JS [1 ]
机构：
[1] USDA ARS,BELTSVILLE AGR RES CTR,FRUIT LAB,BELTSVILLE,MD 20705
来源：
CURRENT MICROBIOLOGY | 1995年 / 31卷 / 02期
关键词：
D O I：
10.1007/BF00294290
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Genetic relationships among 11 Xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic DNA (RAPD). Twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. Phenetic analysis based on a similarity matrix corresponded well with previous reports on X. fastidiosa RFLP-based similarity relationships, indicating that RAPD-PCR amplification products can be used as a reliable indicator of genetic distance in X. fastidiosa. Cladistic analysis suggests the existence of five groups of X. fastidiosa: the citrus group, the plum-elm group, the grape-ragweed group, the almond group, and the mulberry group.
引用
收藏
页码：134 / 137
页数：4
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