APPLICATION OF SPHEROID CULTURE TO HUMAN HEPATOCYTES AND MAINTENANCE OF THEIR DIFFERENTIATION

被引：58
|
作者：
TONG, JZ
SARRAZIN, S
CASSIO, D
GAUTHIER, F
ALVAREZ, F
机构：
[1] INSERM,U347,F-94276 LE KREMLIN BICETR,FRANCE
[2] INST CURIE,CNRS,URA 1343,F-91405 ORSAY,FRANCE
来源：
BIOLOGY OF THE CELL | 1994年 / 81卷 / 01期
关键词：
HUMAN HEPATOCYTE; SPHEROID CULTURE; DIFFERENTIATION; CYTOCHROME P-450;
D O I：
10.1016/0248-4900(94)90058-2
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Human hepatocytes cultured with a hormonally defined medium on non-adherent poly-(2-hydroxyethyl methacrylate)coated surface were able to form spheroids. The maintenance of liver-specific functions was assessed by following secretion of albumin, transferrin and al-antitrypsin that were still detectable after 4 months of spheroidal culture. Moreover, cytochrome P-450 IA was induced by methylcholanthrene for up to 2 weeks. This cell system is very promising for long-term in vitro studies of human hepatocyte functions.
引用
收藏
页码：77 / 81
页数：5
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