ALTERATION OF THE T-CELL REPERTOIRE AFTER BONE-MARROW TRANSPLANTATION

We investigated T cell receptor (TCR) alpha/beta and gamma/delta repertoire reconstitution after autologous and allogeneic bone marrow transplantation (BMT) in humans using 13 monoclonal antibodies directed at constant and variable regions of the TCR. The TCR gamma/delta repertoire was studied kinetically during the first month and then 1 year post-BMT whereas alpha/beta peripheral blood T lymphocytes (PBTL) mere studied within the first 3 months and 1 year post-BMT. Through these two studies, we found 7 of 26 patients with over-represented TCR gamma/delta subsets during the early period post-BMT. Moreover, during this period the V gamma 9V delta 2 frequency among gamma/delta T cells was found to be higher than among normal donors. Study on TCR alpha/beta T cells also revealed abnormally expanded V-specific subset (5 of 10 patients within 3 months following BMT) demonstrating that repertoire alteration early after BMT is a general phenomenon concerning potentially all T cell subsets. More surprisingly, the T cell repertoire was also found to be altered Late after BMT (7 of 15 patients after 1 year post-BMT presented one or more overepresented alpha/beta TCR subset). These alterations of TCR combinatorial diversity should be taken into account in understanding the immunological status of patients after BMT.