Hemato-biochemical and oxidative effect of fresh juice and ethanolic extract of Ficus tsiela Roxb in rats

Aim: The goal was to assess the hemato-biochemical and oxidative changes of Ficus tsiela Roxb in rats. Materials and Methods: A total of 48 adult female Sprague-Dawley rats weighing 200-250 g were divided into six groups with eight rats in each group. Group 1 received no treatment served as a control group. Group 2 and 3 animals were orally ingested with fresh juice from 5 g of leaves and 10 g of leaves, respectively. Group 4, 5 and 6 animals were orally administered with the extract at doses of 750, 1000 and 1500 mg/kg, respectively. The experiment was continued for a period of 21 days. Biochemical parameters including aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatinine kinase (CK), creatinine and blood glucose and hematological parameters such as hemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC) and differential leucocyte count (DLC) were determined on 0, 7, 14, and 21 day of treatment. Lipid peroxides and reduced glutathione values were determined in liver at day 21. Results: Hb, PCV, TLC and DLC showed no significant variations in treated groups compared with control and ALP, AST, CK and creatinine values showed a significant increase in serum of the higher dose groups. There was no variation in the level of blood sugar. There was an increase in the values of lipid peroxides and reduction in the values of reduced glutathione in the liver homogenate which indicated oxidative damage. Conclusion: The fresh juice and ethanolic extract at higher doses had no effect on hematological values. However, biochemical parameters affected adversely. Both fresh juice and extract caused oxidative damage to liver.