DETECTION OF GENOMIC ALTERATIONS IN CARCINOGEN-INDUCED MOUSE-LIVER TUMORS BY DNA FINGERPRINT ANALYSIS

被引：8
|
作者：
MULLER, O
ALONSO, A
BUCHMANN, A
BAUERHOFMANN, R
BOCK, KW
SCHWARZ, M
机构：
[1] GERMAN CANC RES CTR,INST EXPTL PATHOL,NEUENHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY
[2] UNIV TUBINGEN,INST TOXICOL,W-7400 TUBINGEN 1,GERMANY
来源：
MOLECULAR CARCINOGENESIS | 1990年 / 3卷 / 06期
关键词：
HEPATOCARCINOGENESIS; LIVER TUMORS; DNA FINGERPRINTING;
D O I：
10.1002/mc.2940030604
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA fingerprint analysis was used to study structural abnormalities in the genome of mouse liver tumor cells. Liver tumors were induced in three strains of mice, namely C57BL/6J, C3H/He and B6C3F1, by a single injection of 20-mu-g/g body wt. diethylnitrosamine on day 15 after birth. DNA from liver tumors was digested with Hinfl restriction enzyme and hybridized on Southern blots with wild-type bacteriophage M13 DNA as probe. The resulting fingerprints of tumor DNA were compared with those of DNA from normal liver tissue. Genomic aberrations were detected in two out of 68 tumors analyzed, one stemming from a C57BL/6J and the other from a C3H/He mouse.
引用
收藏
页码：330 / 334
页数：5
相关论文
共 50 条
  • [31] INCREASED SENSITIVITY FOR DETECTION OF CARCINOGEN-INDUCED DNA-REPAIR WITH CHAIN TERMINATOR DIDEOXYTHYMIDINE
    SMITH, GJ
    CHARLTON, RK
    GRISHAM, JW
    KAUFMAN, DG
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 83 (04) : 1538 - 1544
  • [32] UNSCHEDULED DNA-SYNTHESIS IN CARCINOGEN-TREATED HUMAN AND MOUSE-LIVER CELLS AND FIBROBLASTS
    BELITSKY, GA
    BUDANOVA, IV
    [J]. IARC PUBLICATIONS, 1983, (51): : 123 - 133
  • [33] ANALYSIS BY BENZOYLATED-DEAE-CELLULOSE OF CARCINOGEN-INDUCED DNA DAMAGE INVIVO
    STEWART, BW
    HUANG, PHT
    [J]. PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1977, 18 (MAR): : 23 - 23
  • [34] EFFECTS OF ALTERATIONS IN EARLY HORMONAL ENVIRONMENT ON DEVELOPMENT AND HORMONE DEPENDENCY OF CARCINOGEN-INDUCED MAMMARY-TUMORS IN RATS
    SYLVESTER, PW
    AYLSWORTH, CF
    VANVUGT, DA
    MEITES, J
    [J]. CANCER RESEARCH, 1983, 43 (11) : 5342 - 5346
  • [35] Rad18 mediates specific mutational signatures and shapes the genomic landscape of carcinogen-induced tumors in vivo
    Lou, Jitong
    Yang, Yang
    Gu, Qisheng
    Price, Brandon A.
    Qiu, Yuheng
    Fedoriw, Yuri
    Desai, Siddhi
    Mose, Lisle E.
    Chen, Brian
    Tateishi, Satoshi
    Parker, Joel S.
    Vaziri, Cyrus
    Wu, Di
    [J]. NAR CANCER, 2021, 3 (01): : 1 - 18
  • [36] SEQUENTIAL-ANALYSIS OF DNA DAMAGE AND REPAIR DURING THE DEVELOPMENT OF CARCINOGEN-INDUCED RAT-LIVER HYPERPLASTIC LESIONS
    BRAMBILLA, G
    MARTELLI, A
    PINO, A
    ROBBIANO, L
    [J]. CANCER RESEARCH, 1986, 46 (07) : 3476 - 3481
  • [37] A carcinogen-induced mouse model recapitulates the molecular alterations of human muscle-invasive bladder cancer.
    Fantini, Damiano
    Glaser, Alexander P.
    Kalen, Rimar J.
    Yiduo, Wang
    Yu, Yanni
    Meeks, Joshua J.
    [J]. CANCER RESEARCH, 2018, 78 (10) : 69 - 69
  • [38] INDIRECT ALKYLATION OF CBA MOUSE-LIVER DNA AND RNA BY HYDRAZINE INVIVO - A POSSIBLE MECHANISM OF ACTION AS A CARCINOGEN
    QUINTERORUIZ, A
    PAZNERI, LL
    VILLATREVINO, S
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1981, 67 (03) : 613 - 618
  • [39] GENOMIC HYPOMETHYLATION AND FAR-5' SEQUENCE ALTERATIONS ARE ASSOCIATED WITH CARCINOGEN-INDUCED ACTIVATION OF THE HAMSTER THYMIDINE KINASE GENE
    BARR, FG
    RAJAGOPALAN, S
    MACARTHUR, CA
    LIEBERMAN, MW
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (09) : 3023 - 3033
  • [40] ANALYSIS OF MAMMALIAN DNA FOR THE PRESENCE OF CARCINOGEN-INDUCED PHOSPHOTRIESTERS - APPLICATION OF THE TECHNIQUE OF DIFFERENCE SEDIMENTATION
    SHOOTER, KV
    MERRIFIELD, RK
    [J]. ANALYTICAL BIOCHEMISTRY, 1980, 103 (01) : 110 - 117
12345