DETECTION OF GENOMIC ALTERATIONS IN CARCINOGEN-INDUCED MOUSE-LIVER TUMORS BY DNA FINGERPRINT ANALYSIS

被引：8
|
作者：
MULLER, O
ALONSO, A
BUCHMANN, A
BAUERHOFMANN, R
BOCK, KW
SCHWARZ, M
机构：
[1] GERMAN CANC RES CTR,INST EXPTL PATHOL,NEUENHEIMER FELD 280,W-6900 HEIDELBERG,GERMANY
[2] UNIV TUBINGEN,INST TOXICOL,W-7400 TUBINGEN 1,GERMANY
来源：
MOLECULAR CARCINOGENESIS | 1990年 / 3卷 / 06期
关键词：
HEPATOCARCINOGENESIS; LIVER TUMORS; DNA FINGERPRINTING;
D O I：
10.1002/mc.2940030604
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA fingerprint analysis was used to study structural abnormalities in the genome of mouse liver tumor cells. Liver tumors were induced in three strains of mice, namely C57BL/6J, C3H/He and B6C3F1, by a single injection of 20-mu-g/g body wt. diethylnitrosamine on day 15 after birth. DNA from liver tumors was digested with Hinfl restriction enzyme and hybridized on Southern blots with wild-type bacteriophage M13 DNA as probe. The resulting fingerprints of tumor DNA were compared with those of DNA from normal liver tissue. Genomic aberrations were detected in two out of 68 tumors analyzed, one stemming from a C57BL/6J and the other from a C3H/He mouse.
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收藏
页码：330 / 334
页数：5
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