PHOTOLITHOGRAPHICALLY PATTERNED ENZYME MEMBRANES FOR THE DETECTION OF PESTICIDES AND COPPER(II) BASED ON ENZYME-INHIBITION

被引：21

作者：

ZURN, A ^{[1
]}

MULLER, H ^{[1
]}

机构：

[1] UNIV HALLE,FACHBEICH CHEM,INST ANALYT & UMWELTCHEM,GEUSAER STR,O-4200 MERSEBURG,GERMANY

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1993年 / 346卷 / 6-9期

关键词：

D O I：

10.1007/BF00321251

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A non-aqueous and an aqueous photopolymer system with an enzyme are used to prepare photolithographically patterned enzyme membranes for amperometric (thinfilm platinum electrode) and potentiometric (ISFET) sensors based on enzyme inhibition. Flow methods for enzyme inhibition tests are described. The decrease in enzyme (AChE) activity after incubation in a solution of dichlorvos as inhibitor is detected amperometrically. The enzyme urease is immobilized onto the pH-sensitive gate area of an ISFET. Such a biosensor is able to detect copper(II) in water in the ppm-range without preconcentration.

引用

页码：589 / 593

页数：5

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