SYNTHESIS AND SECRETION OF HUMAN ATRIAL-NATRIURETIC-PEPTIDE IN ESCHERICHIA-COLI

被引：0
|
作者：
NIKOLAIDIS, MN
PARSADANYAN, AS
POZMOGOVA, GE
ELDAROV, MA
ANANEVA, NM
MAISURYAN, NA
GALOYAN, AA
机构：
[1] RUSSIAN ACAD SCI,CTR BIOENGN,MOSCOW 117334,RUSSIA
[2] RUSSIAN ACAD MED SCI,CARDIOL RES CTR,MOSCOW 121552,RUSSIA
来源：
MOLECULAR BIOLOGY | 1994年 / 28卷 / 05期
关键词：
ATRIAL NATRIURETIC PEPTIDE; EXPRESSION; ST AUREUS PROTEIN A;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A recombinant plasmid was constructed to provide synthesis in E. coli of the atrial natriuretic peptide (ANP) fused with Staphylococcus aureus protein A. Secretion and proteolytic stability of the hybrid protein was examined in different recipient strains acid different cultivation conditions. The hybrid protein was purified on IgG Sepharose. The presence of ANP in the hydrolysate was confirmed with immunoenzyme assay and analytical HPLC.
引用
收藏
页码：704 / 708
页数：5
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