Comparison of Background Corrected Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Correlation Spectroscopy: Dilution Series Revisited

被引：1
|
作者：
Ruettinger, Steffen [1 ]
Kapusta, Peter [2 ]
Patting, Matthias [2 ]
Wahl, Michael [2 ]
Macdonald, Rainer [1 ]
机构：
[1] Phys Tech Bundesanstalt, Abbestr 2-12, D-10587 Berlin, Germany
[2] PicoQuant GmbH, D-12489 Berlin, Germany
来源：
SINGLE MOLECULE SPECTROSCOPY AND IMAGING II | 2009年 / 7185卷
关键词：
CONFOCAL VOLUME; TIME; SENSITIVITY; ACCURACY; SYSTEM;
D O I：
10.1117/12.808883
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We performed quantitative tests in order to compare the practical limits of FCS and FLCS. Unlike conventional FCS, FLCS yields precise and correct concentration values from as low as picomolar to micromolar concentrations. We discuss some of the inherent technical limitations of FCS and demonstrate that they are easily overcome by FLCS employing the simplest confocal detection scheme.
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页数：6
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