Fluorescence lifetime correlation spectroscopy

被引:1
|
作者
Jana, Humpolickova [1 ]
Jan, Sykora [1 ]
Petr, Kapusta [2 ]
Michael, Wahl [2 ]
Ales, Benda [1 ]
Joerg, Enderlein [3 ]
Martin, Hof [1 ]
机构
[1] Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, Dolejskova 3, CR-18223 Prague 8, Czech Republic
[2] PicoQuant GmbH, D-12489 Berlin, Germany
[3] Forschungszentrum Julich, Inst Biol Informat Proc 1, D-52425 Julich, Germany
关键词
D O I
10.1117/12.736637
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Fluorescence lifetime correlation spectroscopy (FLCS) is a recently developed method which combines the conventional fluorescence correlation spectroscopy (FCS) and time correlated single photon counting (TCSPC). It enables to perform a signal separation of the species which possess different lifetime. Particular diffusion components of a mixture of more fluorescent species can be thus separated. Moreover, the detector afterpulsing can be suppressed by FLCS.
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收藏
页数:9
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