QUANTITATION OF URINARY HYDROXYPYRIDINIUM CROSS-LINKS FROM COLLAGEN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：45
|
作者：
KOLLERUP, G
THAMSBORG, G
BHATIA, H
SORENSEN, OH
机构：
[1] SUNDBY HOSP,DEPT MED,DK-2300 COPENHAGEN S,DENMARK
[2] SUNDBY HOSP,DEPT CLIN CHEM,DK-2300 COPENHAGEN S,DENMARK
来源：
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1992年 / 52卷 / 07期
关键词：
COLLAGEN DEGRADATION; DEOXYPYRIDINOLINE; HPLC; PYRIDINOLINE; URINARY CROSS-LINKS;
D O I：
10.3109/00365519209115510
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Pyridinoline and deoxypyridinoline are intermolecular cross-links in mature collagen in bone and cartilage. The urinary excretion of the two compounds correlates well to bone turnover. A fast, sensitive, and accurate isocratic ion-pairing reverse-phase high-performance liquid chromatography method for measurement of pyridinoline and deoxypyridinoline in urine has been established. Intra- and inter-assay precision were 5-7% and 12-14%, respectively. Recovery for pyridinoline was 97.4% and for deoxypyridinoline 94.3%. The detection limit was 0.4 pmol. Pyridinoline:creatinine and deoxypyridinoline: creatinine ratios in healthy subjects, were 38.8 nmol:mmol and 13.0 nmol: mmol, respectively. Increased values of both cross-links were observed in children, in the age group 20-29 in both sexes, and in post-menopausal women.
引用
收藏
页码：657 / 662
页数：6
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