HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ANALYSIS OF 8-METHOXYPSORALEN MONOADDUCTS AND CROSS-LINKS IN LYMPHOCYTES AND KERATINOCYTES

被引：22
|
作者：
BEVILACQUA, PM [1 ]
EDELSON, RL [1 ]
GASPARRO, FP [1 ]
机构：
[1] YALE UNIV,SCH MED,DEPT DERMATOL,PHOTOBIOL LAB,333 CEDAR ST,POB 3333,NEW HAVEN,CT 06510
来源：
JOURNAL OF INVESTIGATIVE DERMATOLOGY | 1991年 / 97卷 / 01期
关键词：
D O I：
10.1111/1523-1747.ep12479321
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
Human lymphocytes and murine keratinocytes were treated with clinically relevant doses of 8-methoxypsoralen and long-wavelength ultraviolet radiation. The extent of 8-MOP photoadduct formation was determined by liquid scintillation analysis. The distribution of photoadducts was determined by HPLC analysis of enzymatically hydrolyzed DNA from these cells. Analysis of photoadduct formation in lymphocytes and keratinocytes showed that 4',5'-monoadducts were the predominant photoadducts (42% and 41%, respectively). Monoadduct and crosslink yields were dependent on cell type as well as irradiating wavelength. The photochemical conversion of 400 nm-induced 4',5'-monoadducts to crosslinks, as well as 3,4-monoadducts in poly(dA-dT), was measured.
引用
收藏
页码：151 / 155
页数：5
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