Aim of study: In this study, it was aimed to research the anatomical properties, chemical composition of the cell wall and fiber characteristics of Turkish Red Pine (Pinus brutia Ten.) wood sampled from five different zone of Kastamonu province in Turkey. Area of study: This study was performed at the laboratories of Department of Forest Industry Engineering and Department of Biology, Kastamonu University. Material and Methods: Turkish Red Pine (Pinus brutia Ten.) wood samples obtained from Hanonu, Bozkurt, Arac, Inebolu and Tosya were used as experimental materials. Anatomical properties, chemical structure of wood and fibrous characteristics of wood fibers were determined according to standard methods. Main results: This study indicated that Tosya samples had maximum fibre length with 3.15 mm and maximum lumen width with 27.75. m, whereas maximum fibre width was found in Inebolu samples with 42.27. m and maximum cell wall thickness was seen in samples of Inebolu with 7.90. m. As a consequence of chemical analyses, it was confirmed that holocellulose, alpha-cellulose and lignin ratios, 1% NaOH, ethanol, hot water and cold water solubilities and ash ratio changed within the range of 70.37-72.38, 42.62-47.30, 26.04-27.86, 12.38-13.14, 2.38-3.41, 2.86-3.83, 2.29-3.27 and 0.26-0.40 as percent respectively. In the results of anatomical research, it was seen that Hanonu samples had maximum average ray numbers with 7. Likewise, maximum tracheid widths were found samples in Inebolu and Bozkurt with 1.31 mu m. Besides, maximum earlywood width was confirmed Bozkurt samples with 0.47 mm, whereas maximum latewood width was confirmed Hanonu samples with 1.31 mm. Research highlights: It is inferred that the same tree species taken from different areas have varied anatomical, chemical and fibre properties measured by means of this study. Because of this, it can be stated that growth location properties and growth media affect the characteristics of same type trees.