THE PROTEIN PRODUCT OF THE C-CBL ONCOGENE RAPIDLY COMPLEXES WITH THE EGF RECEPTOR AND IS TYROSINE-PHOSPHORYLATED FOLLOWING EGF STIMULATION

The cbl oncogene was first identified as part of a transforming retrovirus which arose in a mouse pre-B cell lymphoma. Its protein product, p120(cbl): is cytoplasmic and has several distinctive domains including a highly basic region, a RING finger motif and a large proline-rich domain, A mutation to cbl in the 70Z/3 pre-B cell lymphoma produces an oncogenic protein which exhibits a marked enhancement of tyrosine phosphorylation, Parallel studies have demonstrated that p120(cbl) is a substrate of protein tyrosine kinases activated by engagement of the T cell antigen receptor and that cbl is phosphorylated by oncogenic forms of the Abl tyrosine kinase, A genetic analysis of the Caenorhabditis elegans c61 homologue, sli-1, demonstrates that sli-1 negatively regulates the LET-23 tyrosine kinase receptor, Here we show that p120(cbl) is rapidly phosphorylated on tyrosine residues following EGF stimulation and that it forms an inducible complex with the receptor, Our results also show that the oncogenic 70Z/3 form of cbl has enhanced binding to the EGF receptor and that peptides spanning the proline-rich region bind a range SH3 domains. These findings are consistent with a conserved role for cbl/sli-1 proteins in mammals and nematodes.