RANGAP1 INDUCED GTPASE ACTIVITY OF NUCLEAR RAS-RELATED RAN

被引：444

作者：

BISCHOFF, FR ^{[1
]}

KLEBE, C ^{[1
]}

KRETSCHMER, J ^{[1
]}

WITTINGHOFER, A ^{[1
]}

PONSTINGL, H ^{[1
]}

机构：

[1] MAX PLANCK INST MOLEK PHYSIOL,STRUKTURELLE BIOL ABT,D-44139 DORTMUND,GERMANY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 07期

关键词：

GTPASE-ACTIVATING PROTEIN; RCC1; TC4; G(2) CHECKPOINT;

D O I：

10.1073/pnas.91.7.2587

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The nuclear Ras-related protein Ran binds guanine nucleotide and is involved in cell cycle regulation. Models of the signal pathway predict Ran to be active as Ran-GTP at the initiation of S phase upon activation by the nucleotide exchange factor RCC1 and to be inactivated for the onset of mitosis by hydrolysis of bound GTP. Here a nuclear homodimeric 65-kDa protein, RanGAP1, is described, which we believe to be the immediate antagonist of RCC1. It was purified from HeLa cell lysates and induces GTPase activity of Ran, but not Ras, by more than 3 orders of magnitude. The Ran mutant Q69L, modeled after RasQ61L, which is unable to hydrolyze bound GTP, is insensitive to RanGAP1.

引用

页码：2587 / 2591

页数：5

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