TUMOR-DERIVED MONOCYTE CHEMOATTRACTANT PROTEIN-1 INDUCES INTRATUMORAL INFILTRATION OF MONOCYTE-DERIVED MACROPHAGE SUBPOPULATION IN TRANSPLANTED RAT-TUMORS

By immunohistochemistry using anti-rat macrophage monoclonal antibodies RM-1, ED1, ED2, ED3, TRPM-3, and Ki-M2R, we studied transplanted rat tumors of 9L (rat gliosarcoma), Ad-2 (rat mammary carcinoma), and MT-P (rat malignant fibrous histiocytoma) cell lines to examine the distribution pattern of macrophages within and around the tumors. Most tumor-associated macrophages expressed RM-I, EDI, and In antigens, indicating activated macrophages. Based on differences in their immunophenotypical expression, these macrophages were distinguished into two mafor subpopulations. One expressed TRPM-3 and/or ED3, and the other was positive for ED2 and Ki-M2R The former was considered to be monocyte-derived macrophages, whereas the latter showed the immunophenotype of tissue-fixed, resident macrophages. Infiltration and distribution patterns in the two macrophage subpopulations differed in the three different tumors. Monocyte-derived, activated macrophages infiltrated into 9L- and Ad-2-transplanted tumors, which markedly produced monocyte chemoattractant protein-1 (MCP-1). Additionally, numerous ED2- and Ki-M2R-positive macrophages were observed within the Ad-2-transplanted tumors, and some of them expressed TRPM-3. However, there were few macrophages in the MT-P-transplanted tumors that showed no MCP-1 production In transplanted tumors of four MT-P/MCP-1 cell lines established by transfecting a mt MCP-I gene expression vector (pCEP4/MCP-1) into the MT-P cell line, different levels of MCP-1 production were detected which correlated well with the numbers of intratumorally infiltrated TRPM-3-positive macrophages. In contrast, ED2- and Ki-M2R-positive macrophages were not detected in any MT-P/MCP-1-transplanted tumors. MT-P/ MCP-1-transplanted tumors exhibited lower growth rate than parental MT-P-transplanted tumors. These results indicate that tumor-derived MCP-1 induces intratumoral infiltration of monocyte-derived macrophages, but not macrophages with the immunophenotype of tissue-fixed, resident type. The former poplantion of macrophages seems to have a suppuessive effect on the growth of tumors.x