THE SYNTHESIS AND CHARACTERIZATION OF URIDINE 5'-(BETA-L-RHAMNOPYRANOSYL DIPHOSPHATE) AND ITS ROLE IN THE ENZYMATIC-SYNTHESIS OF RUTIN

Uridine 5′-(β-l-rhamnopyranosyl diphosphate) was synthesized by the condensation of uridine 5′-diphenylpyrophosphate and β-l-rhamnopyranosyl phosphate. That sugar 1-phosphate was made via the phosphitylation of the hemiacetal hydroxyl group of 2,3,4-tetra-O-acetyl-β-l-rhamnopyranose. An enzyme preparation from the primary leaves of mung bean (Phaseolus aureus) was shown to catalyze the transfer of l-rhamnose from UDP-β-l-rhamnose to the flavonol d-glucoside isoquercitrin to form rutin. © 1991.