DETERMINATION OF SERUM OXALATE USING PEROXYOXALATE CHEMILUMINESCENCE OF FREE OXALIC-ACID

被引：13
|
作者：
ALBRECHT, S
HORNAK, H
FREIDT, T
BOHM, WD
WEIS, K
REINSCHKE, A
机构：
[1] MED ACAD CARL GUSTAV CARUS,DIAGNOST LAB,O-8019 DRESDEN,GERMANY
[2] MED ACAD CARL GUSTAV CARUS,CLIN MED,DEPT NEPHROL,O-8019 DRESDEN,GERMANY
[3] MED ACAD CARL GUSTAV CARUS,UROL CLIN,O-8019 DRESDEN,GERMANY
来源：
JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1993年 / 8卷 / 01期
关键词：
CHEMILUMINESCENCE; HEMODIALYSIS; OXALATE DETERMINATION; UROLITHIASIS; PEROXYOXALATE CHEMILUMINESCENCE; ASCORBIC ACID;
D O I：
10.1002/bio.1170080105
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a new sensitive and specific method for determination of oxalate in human serum. By using the chemiluminescence decay of monoperoxyoxalic acid very low concentrations of oxalate (200 nmol/L) can be determined. The mean serum oxalate level in apparently healthy controls was 14.5 +/- 8.5 mumol/L. Supplementation of ascorbic acid leads to an increase in serum oxalate level. While serum oxalate concentrations of calcium oxalate stone formers (x = 16.4 +/- 9.8 mumol/L) are not significantly different from the control group, an extreme increase of serum oxalate is evident in haemodialysis patients. The serum oxalate concentration decreased during dialysis treatment from 141.4 +/- 32.1 mumol/L to 36.4 +/- 12.7 mumol/L.
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页码：21 / 24
页数：4
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