Dynamic response of polysilicon microcantilevers to enzymatic hydrolysis of urea

被引：0
|
作者：
Fernandez, Renny Edwin [1 ]
Bhattacharya, Enakshi [1 ]
Chadha, Anju [2 ]
机构：
[1] Indian Inst Technol, Dept Elect Engn, Microelect & MEMS Lab, Madras 600036, Tamil Nadu, India
[2] Indian Inst Technol, Dept Biotechnol, Natl Ctr Catalysis Res, Madras 600036, Tamil Nadu, India
来源：
INTERNATIONAL JOURNAL OF ADVANCES IN ENGINEERING SCIENCES AND APPLIED MATHEMATICS | 2010年 / 2卷 / 1-2期
关键词：
Biosensor; Microcantilever; Resonance frequency; Urea; Urease; Vibrometer;
D O I：
10.1007/s12572-010-0007-6
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Micromolar levels of urea [1-20 mM] on surface micromachined polysilicon cantilever beams immobilized with the enzyme urease was studied. Urea hydrolyses to ammonia and carbon dioxide which are volatile in nature. Monitoring the change in resonance frequency of a urease immobilized cantilever beam during the course of urea hydrolysis, mediated by the immobilized enzyme can give us information on the reaction rates. The change in the resonance frequency of the cantilever beams as a result of urea hydrolysis was measured using Doppler Vibrometry. The reaction kinetics of the enzyme urease was studied by analyzing the dynamic response of the enzyme immobilized microcantilevers for various concentrations of urea.
引用
收藏
页码：17 / 22
页数：6
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