Prostate cancer is the leading cause of cancer among Nigerian men. This study assessed the knowledge, awareness, and screening practices among older men regarding prostate cancer in Oyo State, Nigeria. A cross-sectional study used a multi-stage sampling technique to select 561 adult males. A semi-structured questionnaire was used. Respondents' median age is 60.0 years. Prostate cancer awareness was high [449 (80.0%)]. The overall mean knowledge of prostate cancer causation, treatment, and prevention was 5.8 (+/- 3.0) out of a maximum of 16. Only 109 (19.4%) perceived themselves at risk of developing prostate cancer, but only 4.5% have ever been screened. Though knowledge and risk perception of prostate cancer were low, a majority of respondents (81.5%) were willing to be screened for the disease. Community-based prostate cancer educational interventions and provision of screening centers are needed for this group.