EFFICIENT PRODUCTION OF BACILLUS-STEAROTHERMOPHILUS ALPHA-AMYLASE IN BACILLUS-BREVIS BY ALTERING ITS SIGNAL PEPTIDE

被引：0
|
作者：
YAMAGUCHI, K [1 ]
UEDA, M [1 ]
UDAKA, S [1 ]
机构：
[1] NAGOYA UNIV, FAC AGR, DEPT FOOD SCI & TECHNOL, NAGOYA 46401, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 08期
关键词：
D O I：
10.1271/bbb.57.1384
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To investigate the role of signal peptides in extracellular production in Bacillus brevis, the Bacillus stearothermophilus alpha-amylase signal peptide was systematically altered and the effects were analyzed in B. brevis. Efficient extracellular production of the amylase in B. brevis was accomplished either by introducing point mutations at positions between -6 and -4 or by replacing the whole signal peptide with that of B. brevis middle wall protein.
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页码：1384 / 1386
页数：3
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