PROTEIN KINASE-A MODULATES CA2+-DEPENDENT AND PROTEIN KINASE-C-DEPENDENT AMYLASE RELEASE IN PERMEABILIZED RAT PANCREATIC ACINI

被引：25

作者：

OSULLIVAN, AJ ^{[1
]}

JAMIESON, JD ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT CELL BIOL,C222 SHM,333 CEDAR ST,NEW HAVEN,CT 06510

来源：

BIOCHEMICAL JOURNAL | 1992年 / 287卷

关键词：

D O I：

10.1042/bj2870403

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of protein kinase A (PKA) in the release of amylase from permeabilized pancreatic acini was investigated. Addition of cyclic AMP (cAMP) to permeabilized acini resulted in a potentiation of Ca2+-dependent amylase release, shifting the Ca2+ dose/response curve leftwards. As with protein kinase C (PKC) activation, this is due to an increase in the time of active discharge. The effect of cAMP was shown to be blocked by two inhibitors of PKA, H89 and the PKI-(5-24)-peptide. At low concentration, cAMP synergizes from phorbol 12-myristate 13-acetate (PMA), while at optimal concentrations cAMP and PMA are additive. PKA and PKC appear to work via similar, but not identical mechanisms.

引用

页码：403 / 406

页数：4

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