USE OF BACTERIOPHAGE FOR THE SELECTIVE ISOLATION OF THERMOPHILIC ACTINOMYCETES FROM COMPOSTED EUCALYPTUS BARK

被引：13
|
作者：
KURTBOKE, DI
MURPHY, NE
SIVASITHAMPARAM, K
机构：
[1] University of Western Australia, School of Agriculture, Soil Science and Plant Nutrition, Nedlands
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 1993年 / 39卷 / 01期
关键词：
BACTERIOPHAGE; THERMOPHILIC BACTERIA; THERMOPHILIC ACTINOMYCETES; COMPOSTED EUCALYPTUS BARK;
D O I：
10.1139/m93-007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method was developed to reduce the numbers of thermophilic bacteria on isolation plates, which in turn facilitated the detection and isolation of thermophilic actinomycetes. The method involves exposing the test material to bacteriophage suspensions prior to inoculation on isolation plates. This method was applied to composted eucalyptus bark samples, which were then inoculated on R8 and 1/2 TSA + 0.2% casein hydrolysate agar plates. The phage susceptibility of thermophilic bacteria provided a selective means of reducing their numbers on isolation plates and hence increased the numbers of Thermomonospora, Saccharopolyspora rectivirgula, and thermophilic Streptomyces spp. on these media in comparison with the numbers recorded from control plates.
引用
收藏
页码：46 / 51
页数：6
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