A RAPID METHOD FOR SCREENING VACCINIA VIRUS RECOMBINANTS

被引：0
|
作者：
LAI, ACK [1 ]
CHU, Y [1 ]
机构：
[1] CUNY MT SINAI SCH MED,DEPT MICROBIOL,NEW YORK,NY 10029
来源：
BIOTECHNIQUES | 1991年 / 10卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid and small-scale method for screening vaccinia virus recombinants employing micrococcal nuclease is described. This protocol utilizes the differential sensitivity of cellular and viral DNA to the nuclease, which can be selectively activated by addition of Ca2+ and inactivated by elimination of Ca2+. Two to five micrograms of viral DNA can be obtained from one infected L cell plate (50 mm) after overnight incubation.
引用
收藏
页码：564 / 565
页数：2
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