ISOLATION AND CHARACTERIZATION OF A MULTIENZYME COMPLEX CONTAINING DNA REPLICATIVE ENZYMES FROM MITOCHONDRIA OF SACCHAROMYCES-CEREVISIAE - MULTIENZYME COMPLEX FROM YEAST MITOCHONDRIA

被引：6
|
作者：
MURTHY, V [1 ]
PASUPATHY, K [1 ]
机构：
[1] BHABHA ATOM RES CTR,DIV RADIAT BIOL & BIOCHEM,BOMBAY 400085,MAHARASHTRA,INDIA
来源：
MOLECULAR BIOLOGY REPORTS | 1995年 / 20卷 / 03期
关键词：
MITOCHONDRIA; MULTIENZYME COMPLEX; REPLICATION; SACCHAROMYCES CEREVISIAE;
D O I：
10.1007/BF00990545
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 40 S multienzyme complex containing mtDNA polymerase was isolated from mitochondria of S. cerevisiae by density gradient centrifugation and by gel filtration chromatography. Besides DNA polymerase, RNA polymerase, primase, 3'-->5' exonuclease and an ATPase activities were found to be associated with it. The presence of some of these enzymes were confirmed by Western blot. This high molecular weight multienzyme complex containing DNA has most of the attributes of a putative replisome.
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页码：135 / 141
页数：7
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