DETECTION OF IRON-BINDING PROTEINS BY A BLOTTING TECHNIQUE

被引：13
|
作者：
CHEN, Y
DRYSDALE, J
机构：
[1] Department of Biochemistry, Tufts University School of Medicine, Boston
来源：
ANALYTICAL BIOCHEMISTRY | 1993年 / 212卷 / 01期
关键词：
D O I：
10.1006/abio.1993.1289
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a simple method for detecting iron binding proteins based on binding of 59Fe to protein blots from SDS gels followed by autoradiography. This binding is specific and is not affected by other metal ions. The method has allowed identification of new iron binding proteins in intestinal mucosa and in Hela cells. © 1994 Academic Press, Inc. All rights reserved.
引用
收藏
页码：47 / 49
页数：3
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