DUAL EFFECTS OF OPIOIDS ON TRANSMITTER RELEASE, INTRACELLULAR CALCIUM AND CALCIUM-UPTAKE IN SK-N-SH CELL-LINE

被引：4
|
作者：
SARNE, Y
GAFNI, M
FIELDS, A
KEREN, O
机构：
[1] Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv
来源：
REGULATORY PEPTIDES | 1994年 / 54卷 / 01期
关键词：
D O I：
10.1016/0167-0115(94)90485-5
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The effect of opiates on KCl-induced neurotransmitter release (preloaded [H-3] norepinephrine), intracellular calcium level (fura 2) and calcium influx (Ca-45(++) uptake) was measured in the human neuroblastoma SK-N-SH. Opiates exerted either inhibition or augmentation of transmitter release, both effects were blocked by naloxone. Similarly, opiates either reduced or potentiated KCl-induced elevation in cytosolic [Ca++], with neighboring cells responding oppositely. The stimulatory effect of opiates on cytosolic calcium was dependent on the presence of extracellular calcium ions. Opiates stimulated calcium uptake by SK-N-SH cells. This stimulation was blocked by naloxone but was not prevented by pretreating the cells with pertusis toxin.
引用
收藏
页码：253 / 254
页数：2
相关论文
共 50 条
  • [21] Cytotoxicity of hexanedione isomers on the human neuroblastoma cell line SK-N-SH
    Zilz, TR
    Coleman, MD
    TOXICOLOGY, 2004, 202 (1-2) : 134 - 135
  • [22] The effect of an organophosphorus agent on human neuroblastoma cell line SK-N-SH
    Yamada, K.
    Shiraishi, H.
    Nameera, A.
    Arima, Y.
    Nagao, M.
    Yamada, Y.
    JOURNAL OF NEUROCHEMISTRY, 2015, 134 : 147 - 147
  • [23] The neuroprotective effects of estrogen in SK-N-SH neuroblastoma cell cultures
    Ba, F
    Pang, PKT
    Davidge, ST
    Benishin, CG
    NEUROCHEMISTRY INTERNATIONAL, 2004, 44 (06) : 401 - 411
  • [24] Dexamethasone blocks the migration of the human neuroblastoma cell line SK-N-SH
    Casulari, L. A.
    Dondi, D.
    Maggi, R.
    Demissie, M.
    Piccolella, M.
    Piva, F.
    BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2006, 39 (09) : 1233 - 1240
  • [25] Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium
    Wang, Hongbo
    Wang, Xiaolong
    Li, Yan
    Yu, Hao
    Wang, Changliang
    Feng, Chunmei
    Xu, Guohui
    Chen, Jiajun
    You, Jiabin
    Wang, Pengfei
    Wu, Xu
    Zhao, Rui
    Zhang, Guohua
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 15 (04) : 3791 - 3800
  • [26] SENSITIVITY TO 2,5-HEXANEDIONE OF NEUROFILAMENTS IN NEUROBLASTOMA CELL-LINE SK-N-SH INCREASES DURING DIFFERENTIATION
    HEIJINK, E
    BOLHUIS, PA
    DEWOLFF, FA
    JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1995, 54 (01): : 82 - 90
  • [27] Neuronal Uptake and Neuroprotective Effect of Curcumin-Loaded PLGA Nanoparticles on the Human SK-N-SH Cell Line
    Doggui, Sihem
    Sahni, Jasjeet Kaur
    Arseneault, Madeleine
    Dao, Le
    Ramassamy, Charles
    JOURNAL OF ALZHEIMERS DISEASE, 2012, 30 (02) : 377 - 392
  • [28] NCX3 alleviates ethanol-induced apoptosis of SK-N-SH cells via the elimination of intracellular calcium ions
    Xia, Zhixiu
    Wang, Changliang
    Wang, Xiaolong
    Yu, Hao
    Yao, Hui
    Shen, Hui
    Lan, Xinze
    Wu, Xu
    Zhang, Guohua
    TOXICOLOGY IN VITRO, 2021, 72
  • [29] Apoptotic and necrotic effects of hexanedione derivatives on the human neuroblastoma line SK-N-SH
    Zilz, Thomas R.
    Griffiths, Helen R.
    Coleman, Michael D.
    TOXICOLOGY, 2007, 231 (2-3) : 210 - 214
  • [30] ORGANOPHOSPHATE-INDUCED ALTERATIONS IN MUSCARINIC RECEPTOR-BINDING AND PHOSPHOINOSITIDE HYDROLYSIS IN THE HUMAN SK-N-SH CELL-LINE
    KATZ, LS
    MARQUIS, JK
    NEUROTOXICOLOGY, 1992, 13 (02) : 365 - 378
12345