DUAL EFFECTS OF OPIOIDS ON TRANSMITTER RELEASE, INTRACELLULAR CALCIUM AND CALCIUM-UPTAKE IN SK-N-SH CELL-LINE

被引：4
|
作者：
SARNE, Y
GAFNI, M
FIELDS, A
KEREN, O
机构：
[1] Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv
来源：
REGULATORY PEPTIDES | 1994年 / 54卷 / 01期
关键词：
D O I：
10.1016/0167-0115(94)90485-5
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The effect of opiates on KCl-induced neurotransmitter release (preloaded [H-3] norepinephrine), intracellular calcium level (fura 2) and calcium influx (Ca-45(++) uptake) was measured in the human neuroblastoma SK-N-SH. Opiates exerted either inhibition or augmentation of transmitter release, both effects were blocked by naloxone. Similarly, opiates either reduced or potentiated KCl-induced elevation in cytosolic [Ca++], with neighboring cells responding oppositely. The stimulatory effect of opiates on cytosolic calcium was dependent on the presence of extracellular calcium ions. Opiates stimulated calcium uptake by SK-N-SH cells. This stimulation was blocked by naloxone but was not prevented by pretreating the cells with pertusis toxin.
引用
收藏
页码：253 / 254
页数：2
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