DETERMINATION OF URIC-ACID AND XANTHINE HYPOXANTHINE IN BLOOD USING IMMOBILIZED ENZYMES IN FLOW-INJECTION SYSTEM

被引:0
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作者
HUSSAIN, G [1 ]
YAQOOB, M [1 ]
NABI, A [1 ]
MASOOM, M [1 ]
机构
[1] UNIV BALUCHISTAN,INST BIOCHEM,QUETTA,PAKISTAN
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中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A flow injection system containing on-line immobilized enzyme mini-columns of urate oxidase and xanthine oxidase/urate oxidase for the determination of uric acid and xanthine/hypoxanthine is described. For the determination of xanthine and hypoxanthine, a xanthine oxidase column was incorporated on-line prior to the mate oxidase column. The results of the uric acid analysis in serum were compared with the commercial kit method in use in hospitals with a correlation coefficient of 0.99 and r.s.d. of 1% (n = 6). The method is fast and the cost per analysis is greatly reduced.
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页码:109 / 112
页数:4
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