A flow injection system containing on-line immobilized enzyme mini-columns of urate oxidase and xanthine oxidase/urate oxidase for the determination of uric acid and xanthine/hypoxanthine is described. For the determination of xanthine and hypoxanthine, a xanthine oxidase column was incorporated on-line prior to the mate oxidase column. The results of the uric acid analysis in serum were compared with the commercial kit method in use in hospitals with a correlation coefficient of 0.99 and r.s.d. of 1% (n = 6). The method is fast and the cost per analysis is greatly reduced.