ANALYSIS OF BUMETANIDE IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：15
|
作者：
GRADEEN, CY [1 ]
BILLAY, DM [1 ]
CHAN, SC [1 ]
机构：
[1] FOOTHILLS PROV GEN HOSP,DEPT LAB MED,1403 29TH ST NW,CALGARY T2N 2T9,ALBERTA,CANADA
来源：
JOURNAL OF ANALYTICAL TOXICOLOGY | 1990年 / 14卷 / 02期
关键词：
D O I：
10.1093/jat/14.2.123
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Bumetanide is a potent diuretic. Its concentration in urine after therapeutic doses is in the low ng/mL range. A procedure using reversed-phase HPLC with fluorescence detection is described. Using this procedure, the authors have been able to measure bumetanide in a urine sample at 10 ng/mL with a signal-to-noise ratio of 7. Therefore, the detection limit is lower than this concentration. Confirmation is by a GC/MS procedure after methylating the diuretic. Derivatization is by extractive alkylation with tetrabutylammonium hydrogen sulfate as the phase transfer reagent and iodomethane as the methylating agent. © 1990 Oxford University Press.
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页码：123 / 126
页数：4
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