ANALYSIS OF SEPARATION EFFICIENCY IN CAPILLARY ELECTROPHORESIS WITH DIRECT CONTROL OF ELECTROOSMOSIS BY USING AN EXTERNAL ELECTRIC-FIELD

被引：37
|
作者：
LEE, CS
WU, CT
LOPES, T
PATEL, B
机构：
[1] Department of Chemical and Biochemical Engineering, Baltimore, MD 21228, University Maryland Baltimore County Campus
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 559卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(91)80065-O
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Direct control of electroosmosis in capillary electrophoresis with the application of an external electric field is demonstrated by the UV marker method. When the zeta potential at the capillary/aqueous solution interface is small, the capacitor model qualitatively and quantitatively predicts the effectiveness of the external electric field for controlling electroosmosis at different electrolyte concentrations and capillary dimensions. To investigate the separation efficiency of capillary electrophoresis with the direct control of electroosmosis, frontal analysis of dimethyl sulfoxide as a UV marker are examined. There is no measurable additional band broadening induced by the application of an external electric field.
引用
收藏
页码：133 / 140
页数：8
相关论文
共 50 条
  • [1] DIRECT CONTROL OF THE ELECTROOSMOSIS IN CAPILLARY ZONE ELECTROPHORESIS BY USING AN EXTERNAL ELECTRIC-FIELD
    LEE, CS
    BLANCHARD, WC
    WU, CT
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (14) : 1550 - 1552
  • [2] ANALYSIS OF FACTORS AFFECTING DIRECT CONTROL OF ELECTROOSMOSIS IN CAPILLARY ELECTROPHORESIS BY USING AN EXTERNAL ELECTRIC-FIELD
    LEE, CS
    MCMANIGILL, D
    WU, CT
    PATEL, B
    LOPES, T
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1991, 201 : 10 - ANYL
  • [3] FACTORS AFFECTING DIRECT CONTROL OF ELECTROOSMOSIS USING AN EXTERNAL ELECTRIC-FIELD IN CAPILLARY ELECTROPHORESIS
    LEE, CS
    MCMANIGILL, D
    WU, CT
    PATEL, B
    [J]. ANALYTICAL CHEMISTRY, 1991, 63 (15) : 1519 - 1523
  • [4] Theoretical examination of electroosmosis control with external radial electric field in capillary electrophoresis
    Chen, Y
    Zhu, Y
    [J]. ELECTROPHORESIS, 1999, 20 (09) : 1817 - 1821
  • [5] DISPERSION STUDIES OF CAPILLARY ELECTROPHORESIS WITH DIRECT CONTROL OF ELECTROOSMOSIS
    WU, CT
    HUANG, TL
    LEE, CS
    MILLER, CJ
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (05) : 568 - 571
  • [6] Separation and direct photometric determination of inorganic anions by capillary zone electrophoresis using suppressed electroosmosis
    Takayanagi, T
    Wada, E
    Motomizu, S
    [J]. ANALYTICAL SCIENCES, 1996, 12 (04) : 575 - 579
  • [7] EFFECT OF DIRECT CONTROL OF ELECTROOSMOSIS ON PEPTIDE AND PROTEIN SEPARATIONS IN CAPILLARY ELECTROPHORESIS
    WU, CT
    LOPES, T
    PATEL, B
    LEE, CS
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (08) : 886 - 891
  • [8] Electroosmosis and electrophoresis in soft clay under direct current electric field
    Sun, Zhaohua
    Qing, Yuxing
    Zhang, Cheng
    Eugene, Beukes Demarscho
    [J]. HELIYON, 2024, 10 (07)
  • [9] THE EFFECT OF ELECTRIC-FIELD STRENGTH, BUFFER TYPE AND CONCENTRATION ON SEPARATION PARAMETERS IN CAPILLARY ZONE ELECTROPHORESIS
    ISSAQ, HJ
    ATAMNA, IZ
    MUSCHIK, GM
    JANINI, GM
    [J]. CHROMATOGRAPHIA, 1991, 32 (3-4) : 155 - 161
  • [10] Capillary zone electrophoresis with external radial electric field control of electroosmotic flow and its application to the separation of synthetic oligopeptides
    Kasicka, V
    Prusik, Z
    Sazelova, P
    Barth, T
    Brynda, E
    Machova, L
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 772 (1-2) : 221 - 230
12345