DISPERSION STUDIES OF CAPILLARY ELECTROPHORESIS WITH DIRECT CONTROL OF ELECTROOSMOSIS

被引：22
|
作者：
WU, CT
HUANG, TL
LEE, CS
MILLER, CJ
机构：
[1] UNIV MARYLAND,DEPT CHEM & BIOCHEM ENGN,BALTIMORE CTY CAMPUS,CATONSVILLE,MD 21228
[2] UNIV MARYLAND,DEPT CHEM & BIOCHEM,COLL PK,MD 20742
来源：
ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 05期
关键词：
D O I：
10.1021/ac00053a013
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The zeta potential and the electroosmotic flow in capillary electrophoresis can be directly controlled by using a radial electric potential gradient across the capillary wall. Flow profiles and dispersion in the capillary with the direct control of electroosmosis are studied. The values of total spatial variance measured from the experiment are in good agreement with the predictions based on only molecular diffusion. There is no measurable additional dispersion and band broadening induced by the direct control of electroosmosis. A dc short circuit phenomenon at the capillary/solution interface is proposed for explaining our experimental observations.
引用
收藏
页码：568 / 571
页数：4
相关论文
共 50 条
  • [1] EFFECT OF DIRECT CONTROL OF ELECTROOSMOSIS ON PEPTIDE AND PROTEIN SEPARATIONS IN CAPILLARY ELECTROPHORESIS
    WU, CT
    LOPES, T
    PATEL, B
    LEE, CS
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (08) : 886 - 891
  • [2] DIRECT CONTROL OF THE ELECTROOSMOSIS IN CAPILLARY ZONE ELECTROPHORESIS BY USING AN EXTERNAL ELECTRIC-FIELD
    LEE, CS
    BLANCHARD, WC
    WU, CT
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (14) : 1550 - 1552
  • [3] FACTORS AFFECTING DIRECT CONTROL OF ELECTROOSMOSIS USING AN EXTERNAL ELECTRIC-FIELD IN CAPILLARY ELECTROPHORESIS
    LEE, CS
    MCMANIGILL, D
    WU, CT
    PATEL, B
    [J]. ANALYTICAL CHEMISTRY, 1991, 63 (15) : 1519 - 1523
  • [4] ANALYSIS OF FACTORS AFFECTING DIRECT CONTROL OF ELECTROOSMOSIS IN CAPILLARY ELECTROPHORESIS BY USING AN EXTERNAL ELECTRIC-FIELD
    LEE, CS
    MCMANIGILL, D
    WU, CT
    PATEL, B
    LOPES, T
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1991, 201 : 10 - ANYL
  • [5] ANALYSIS OF SEPARATION EFFICIENCY IN CAPILLARY ELECTROPHORESIS WITH DIRECT CONTROL OF ELECTROOSMOSIS BY USING AN EXTERNAL ELECTRIC-FIELD
    LEE, CS
    WU, CT
    LOPES, T
    PATEL, B
    [J]. JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2): : 133 - 140
  • [6] The role of surfactants in capillary electroosmosis and electrophoresis
    Li, HY
    Gale, RJ
    [J]. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-ENVIRONMENTAL SCIENCE AND ENGINEERING & TOXIC AND HAZARDOUS SUBSTANCE CONTROL, 1996, 31 (09): : 2363 - 2379
  • [7] Theoretical examination of electroosmosis control with external radial electric field in capillary electrophoresis
    Chen, Y
    Zhu, Y
    [J]. ELECTROPHORESIS, 1999, 20 (09) : 1817 - 1821
  • [8] DIRECT CONTROL OF ELECTROOSMOSIS AND RETENTION WINDOW IN MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY
    TSAI, P
    PATEL, B
    LEE, CS
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (10) : 1439 - 1442
  • [9] Separation and direct photometric determination of inorganic anions by capillary zone electrophoresis using suppressed electroosmosis
    Takayanagi, T
    Wada, E
    Motomizu, S
    [J]. ANALYTICAL SCIENCES, 1996, 12 (04) : 575 - 579
  • [10] DYNAMIC SIMULATOR FOR CAPILLARY ELECTROPHORESIS WITH IN-SITU CALCULATION OF ELECTROOSMOSIS
    MOSHER, RA
    ZHANG, CX
    CASLAVSKA, J
    THORMANN, W
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 716 (1-2) : 17 - 26
12345