EFFECT OF TANDEMLY REPEATED AGG TRIPLETS ON THE TRANSLATION OF CAT MESSENGER-RNA IN ESCHERICHIA-COLI

被引：27
|
作者：
IVANOV, I
ALEXANDROVA, R
DRAGULEV, B
SARAFFOVA, A
ABOUHAIDAR, MG
机构：
[1] UNIV TORONTO,DEPT BOT,25 WILLCOCKS ST,TORONTO M5S 1A1,ONTARIO,CANADA
[2] BULGARIAN ACAD SCI,INST MOLEC BIOL,BU-1113 SOFIA,BULGARIA
来源：
FEBS LETTERS | 1992年 / 307卷 / 02期
基金：
加拿大自然科学与工程研究理事会;
关键词：
CAT GENE; GENE EXPRESSION; GENETIC CODE; CODON USAGE; RARE CODONS; SD-SEQUENCE;
D O I：
10.1016/0014-5793(92)80761-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It has been shown that tandems of rare arginine codons AGG have a strong inhibitory effect on translation of mRNA in E coli [5]. This has been explained by the rate-limiting interaction of these codons with the less abundant tRNA(AGG) [6]. In this study tandemly repeated AGG triplets were introduced into the chloramphenicol acetyltransferase (CAT) gene either upstream of the initiation ATG codon or downstream of it (both in frame and out of frame) and the expression of the modified genes was investigated. We report that the addition of AGG clusters resulted in a substantial inhibitory effect on CAT gene expression independently of their localization in mRNA. This inhibitory effect is explained by a competition of the tandem AGGAGG with the natural Shine-Dalgarno (SD) sequence (consensus AAGGAGGU) for the 3'-end of the 16S small ribosomal RNA (rRNA).
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页码：173 / 176
页数：4
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