PROBING THE DISTANCE BETWEEN THE 2 BINDING-SITES OF HIRUDIN FOR ITS INTERACTION WITH THE ACTIVE-SITE AND THE FIBRIN(OGEN)-BINDING SITE OF ALPHA-THROMBIN

被引：0

作者：

WAGNER, U

SCHMITZ, T

OTTE, M

DODT, J

机构：

来源：

BIOMEDICA BIOCHIMICA ACTA | 1991年 / 50卷 / 4-6期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of DELTA(Ser50)-hirudin with alpha-thrombin has been investigated. Deletion of Ser50 of r-hirudin caused a 2.7 fold increase of the K(i) for its complex with alpha-thrombin. Determination of the rate constants k(on) and k(off) for complex formation showed that this effect was mainly due to a change in k(off).

引用

页码：707 / 710

页数：4

共 49 条

[41] ESTIMATION OF THE DISTANCE BETWEEN THE DIVALENT-CATION BINDING-SITE OF DES-1-41-LIGHT CHAIN-ACTIVATED BOVINE PLASMA PROTEIN-C AND A NITROXIDE SPIN LABEL ATTACHED TO THE ACTIVE-SITE SERINE RESIDUE
HILL, KAW
STEINER, SA
CASTELLINO, FJ
[J]. BIOCHEMICAL JOURNAL, 1988, 251 (01) : 229 - 236
[42] INTERACTION OF 2 SULFHYDRYL-REAGENTS WITH A CATION RECOGNITION SITE ON THE NEURONAL DOPAMINE CARRIER EVIDENCES SMALL DIFFERENCES BETWEEN [H-3] GBR-12783 AND [H-3] COCAINE BINDING-SITES
REFAHILYAMANI, F
SAADOUNI, S
COSTENTIN, J
BONNET, JJ
[J]. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1995, 351 (02) : 136 - 145
[43] 5'-(p-fluorosulfonylbenzoyl)-2' (or 3')-(methylanthraniloyl)adenosine, fluorescent affinity labels for adenine nucleotide binding sites: Interaction with the kinase active site of the receptor for epidermal growth factor
Scoggins, RM
Summerfield, AE
Stein, RA
Guyer, CA
Staros, JV
[J]. BIOCHEMISTRY, 1996, 35 (28) : 9197 - 9203
[44] A Highly Conserved Interaction Involving the Middle Residue of the SXN Active-Site Motif Is Crucial for Function of Class B Penicillin-Binding Proteins: Mutational and Computational Analysis of PBP 2 from N. gonorrhoeae
Tomberg, Joshua
Temple, Brenda
Fedarovich, Alena
Davies, Christopher
Nicholas, Robert A.
[J]. BIOCHEMISTRY, 2012, 51 (13) : 2775 - 2784
[45] PREPARATION OF 17-ALPHA-IODOETHYNYLANDROSTA-H, AND 17-ALPHA-(2-IODOETHENYL)ANDROSTA-4,6-DIEN-17-BETA-OL-3-ONES AS ACTIVE-SITE DIRECTED PHOTOAFFINITY LIGANDS FOR ANDROGEN-BINDING PROTEINS
CRUZ, PJD
MASON, NS
DANZO, BJ
SMITH, HE
[J]. STEROIDS, 1992, 57 (11) : 569 - 576
[46] INHIBITION BY TRANEXAMIC ACID OF THE CONVERSION OF SINGLE-CHAIN TISSUE PLASMINOGEN-ACTIVATOR TO ITS 2 CHAIN FORM BY PLASMIN - THE PRESENCE ON TISSUE PLASMINOGEN-ACTIVATOR OF A SITE TO BIND WITH LYSINE BINDING-SITES OF PLASMIN
TAKADA, A
TAKADA, Y
[J]. THROMBOSIS RESEARCH, 1989, 55 (06) : 717 - 725
[47] TOPOGRAPHICAL RELATIONSHIPS AMONG THE MONO-VALENT CATION BINDING-SITES OF BOVINE PLASMA-ACTIVATED PROTEIN-C AND DES(1-41)-LIGHT-CHAIN-ACTIVATED BOVINE PLASMA PROTEIN-C AND A NITROXIDE SPIN LABEL BOUND TO THEIR ACTIVE-SITE SERINE RESIDUES
HILL, KAW
CASTELLINO, FJ
[J]. JOURNAL OF PROTEIN CHEMISTRY, 1987, 6 (06): : 489 - 495
[48] INTERACTION OF THE UNIQUE COMPETITIVE INHIBITOR IMIDAZOLE AND RELATED-COMPOUNDS WITH THE ACTIVE-SITE METAL OF CARBONIC-ANHYDRASE - LINKAGE BETWEEN PH EFFECTS ON THE INHIBITOR BINDING-AFFINITY AND PH EFFECTS ON THE VISIBLE SPECTRA OF INHIBITOR COMPLEXES WITH THE COBALT-SUBSTITUTED ENZYME
KHALIFAH, RG
ROGERS, JI
MUKHERJEE, J
[J]. BIOCHEMISTRY, 1987, 26 (22) : 7057 - 7063
[49] BIOPHYSICAL AND MUTAGENIC ANALYSIS OF A G PROTEIN-COUPLED RECEPTOR: PHOTOCROSSLINKING OF THE TRIDECAPEPTIDE ALPHA-FACTOR INTO STE2P OF SACCHAROMYCES CEREVISIAE REVEALS CONTACT POINTS BETWEEN THE PEPTIDE AND ITS RECEPTOR BINDING SITE
Naider, F.
Son, C.
Sargsyan, H.
Becker, J. M.
[J]. JOURNAL OF PEPTIDE SCIENCE, 2004, 10 : 105 - 105

← 1 2 3 4 5 →