NATIVE GIBBERELLIN-O-GLUCOSIDES FROM MATURE SEEDS OF PHASEOLUS-COCCINEUS

Using an improved purification and derivatization procedure, the endogenous gibberellin-O-glucosides in mature runner beans (Phaseolus coccineus) were analysed by combined gas chromatography-mass spectrometry. In addition to the previously characterized GA(1)-3-O-glucoside and GA(8)-2-O-glucoside, from runner beans, the following GA-O-glucosides were identified as endogenously occurring compounds by comparison of their full scan mass spectra and Kovats retention indices with those of standards: GA(1)-13-O-glucoside, 3-epiGA(1)-3-O-glucoside, GA(5)-13-O-glucoside and GA(29)-2-O-glucoside, The first three are reported in higher plants for the first time. The physiological relevance of the detected GA-O-glucosides is discussed.