FLUORESCENT AND PHOTOAFFINITY-LABELING PROBES FOR RETINOIC ACID RECEPTORS

被引：10
|
作者：
SHIMAZAWA, R
SANDA, R
MIZOGUCHI, H
HASHIMOTO, Y
IWASAKI, S
TANAKA, H
KAGECHIKA, H
SHUDO, K
机构：
[1] UNIV TOKYO,INST APPL MICROBIOL,1-1-1 YAYOI,BUNKYO KU,TOKYO 113,JAPAN
[2] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期
关键词：
D O I：
10.1016/0006-291X(91)91363-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A fluorescent probe for retinoid receptors (RARs) was designed and prepared. The probe consists of a retinoid moiety and a dansyl moiety, i.e., 2-[3-(5-dimethylaminonaphthalene-1-sulfonyl)- aminopropyl-1-oxy]-4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)carboxamido]benzoic acid: DAM-3. DAM-3 specifically bound RARs. Additionally, a photoreactive RAR fluorescent probe was designed and prepared, i.e., 2-[3-(5- azidonaphthalene-1-sulfonyl)aminopropyl-1-oxy]-4-[(5,6,7,8- tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)carboxamido]benzoic acid (ADAM-3). ADAM-3 irreversibly and specifically bound RARs using ultraviolet irradiation. © 1991.
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页码：259 / 265
页数：7
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