EFFECT OF SERA FROM LEAN AND OBESE PIGS ON THE DIFFERENTIATION OF PORCINE ADIPOSE STROMAL-VASCULAR CELLS IN CULTURE

被引：3

作者：

AKANBI, KA ^{[1
]}

HU, CY ^{[1
]}

机构：

[1] OREGON STATE UNIV,DEPT ANIM SCI,CORVALLIS,OR 97331

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1995年 / 111卷 / 02期

关键词：

PORCINE; SERUM; DIFFERENTIATION; CELL CULTURE; ADIPOCYTE; LEAN; OBESE; PIG;

D O I：

10.1016/0300-9629(94)00212-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary cultures of stromal-vascular (S-V) cells from adipose tissue of new-born pigs were used to evaluate the characteristics of four sera obtained from male and female genetically lean or obese pigs weighing 50-60 kg. (2) Lean pig sera (LPS) stimulated greater levels of sn-glycerol-3-phosphate dehydrogenase (GPDH) activity in cultured cells than obese sera (OPS). Male LPS tended to promote higher GPDH specific activity than female LPS or male and female OPS. When GPDH was expressed on a per DNA basis, male LPS significantly (P < 0.05) increased GPDH per unit DNA compared with female LPS or male and female OPS.

引用

页码：293 / 298

页数：6

共 50 条

[31] THE INFLUENCE OF ATTACHMENT PERIOD AND LAMININ ON PREADIPOCYTE DEVELOPMENT IN PORCINE STROMAL-VASCULAR CELL-CULTURE
YU, ZK
HAUSMAN, GJ
WRIGHT, JT
FASEB JOURNAL, 1994, 8 (04): : A21 - A21
[32] Characterization of specific gene expression following differentiation of bovine stromal-vascular cells into adipocytes
Hersom, M. J.
Ross, J. W.
Stein, D.
McBeth, L.
Geisert, R. D.
Desilva, U.
Krebbiel, C. R.
Malayer, J. R.
JOURNAL OF ANIMAL SCIENCE, 2004, 82 : 421 - 421
[33] Characterization of specific gene expression following differentiation of bovine stromal-vascular cells into adipocytes
Hersom, M. J.
Ross, J. W.
Stein, D.
McBeth, L.
Geisert, R. D.
Desilva, U.
Krebbiel, C. R.
Malayer, J. R.
JOURNAL OF DAIRY SCIENCE, 2004, 87 : 421 - 421
[34] Secreted and signaling genes and proteins in fetal and neonatal pig adipose tissue and stromal-vascular cells
Hausman, G. J.
Poulos, S. P.
Richardson, L. R.
Barb, R.
Andacht, T.
Mynatt, R.
Pecot, H.
Crandall, D.
Hreha, A.
POULTRY SCIENCE, 2004, 83 : 200 - 201
[35] Secreted and signaling genes and proteins in fetal and neonatal pig adipose tissue and stromal-vascular cells
Hausman, G. J.
Poulos, S. P.
Richardson, L. R.
Barb, R.
Andacht, T.
Mynatt, R.
Pecot, H.
Crandall, D.
Hreha, A.
JOURNAL OF ANIMAL SCIENCE, 2004, 82 : 200 - 201
[36] DISTRIBUTION OF ADENOSINE METABOLIZING ENZYMES BETWEEN ADIPOCYTE AND STROMAL-VASCULAR CELLS OF ADIPOSE-TISSUE
GREEN, A
NEWSHOLME, EA
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 676 (01) : 122 - 124
[37] Secreted and signaling genes and proteins in fetal and neonatal pig adipose tissue and stromal-vascular cells
Hausman, G. J.
Poulos, S. P.
Richardson, L. R.
Barb, R.
Andacht, T.
Mynatt, R.
Pecot, H.
Crandall, D.
Hreha, A.
JOURNAL OF DAIRY SCIENCE, 2004, 87 : 200 - 201
[38] Characterization of specific gene expression following differentiation of bovine stromal-vascular cells into adipocytes
Hersom, M. J.
Ross, J. W.
Stein, D.
McBeth, L.
Geisert, R. D.
Desilva, U.
Krebbiel, C. R.
Malayer, J. R.
POULTRY SCIENCE, 2004, 83 : 421 - 421
[39] Preferential expression of cholesteryl ester transfer protein mRNA by stromal-vascular cells of human adipose tissue
Radeau, T
Robb, M
McDonnell, M
McPherson, R
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1998, 1392 (2-3): : 245 - 253
[40] Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose
Kim C. O'Connor
Hong Song
Nitsa Rosenzweig
David A. Jansen
Biotechnology Letters, 2003, 25 : 1967 - 1972

← 1 2 3 4 5 →