IN VITRO STUDY OF THE EFFECTIVENESS OF INTRACANAL IRRIGANTS ON CANDIDA ALBICANS

Introduction. Frequently isolated pathogens from teeth With necrotic pulp include various enteric bacteria (Klebsiella, Enterobacter), fungi (especially Candida spp.) and cnterococci (Entcrococcus faccalis). Candida albicans is the most often isolated species. Fungi can be found both in primarily infected teeth and in teeth Where the endodontic treatment has failed. The aim of the current study has been to evaluate the in vitro effectiveness of some of the intracanal irrigants used in eliminating Candida albicans. Materials and Methods. The study used clinical isolates of Candida albicans from infected root canals. The effectiveness of the following endodontic irrigants were studied: 17% EDTA, 2% CHX (chlorhexidine), 6% NaOC1 (sodium hypochlorite), 3% NaOC1 8 3% H2O2 (hydrogen peroxide). The study used an agar-diffusion method: cavities We re made in the agar and then filled with the studied medicaments. The data were input and processed using the statistical package SPSS 15.0.1. The level of significance for rejecting the null hypothesis was fixed at p<0,05. Results. The largest observed zone of inhibition of bacterial growth was achieved with 6% NaOC1 25.88mm and 17% EDTA 24.50mm. The 2% chlorhexidine solution came third in effectiveness compared to the other solutions the zone of inhibition of the bacterial growth was 17.75mm. 3% NaOC1 was less effective than 6%NaOC1, with an inhibition zone of 14.00inm. The lowest effectiveness was registered in the case of 3% H2O2 5.38mm. Conclusions. In infected root canals there are some microorganisms (Candida albicans. E. Faccalis) that are more resistant to chemical and mechanical procedures and this must be taken into account when using various endodontic irrigants and intracanal medicaments. With the reduction of concentration, the effectiveness of NaOC1 against Candida albicans is reduced significantly. The 2% chlorhexidine solution is more effective than 3% NaOC1 in eliminating Candida albicans.