IDENTIFICATION OF OCHRATOXIN-A IN FOOD SAMPLES BY CHEMICAL DERIVATIZATION AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：24
|
作者：
JIAO, YY [1 ]
BLAAS, W [1 ]
RUHL, C [1 ]
WEBER, R [1 ]
机构：
[1] MAX VON PETTENKOFER INST BUNDESGESUNDHEITSAMTES, POB 330013, W-1000 BERLIN 33, GERMANY
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 595卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(92)85183-T
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The contamination of foods with ochratoxin A can be determined verv sensitively by high-performance liquid chromatography (HPLC) with fluorescence detection. A novel procedure is described to confirm OA-positive results quantitatively down to the HPLC detection limit of 0.1 ppb. For this, ochratoxin A in the sample extract is converted into its O-methylochratoxin A methyl ester derivative. which is identified subsequently by gas chromatography mass spectrometry negative-ion chemical ionization and multiple ion detection modes using the hexadeuterated O-methyl-d3-ochratoxin A methyl-d3 ester derivative as internal standard for quantification. In the analysis of more than 60 contaminated samples, the procedure was found to be very accurate.
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页码：364 / 367
页数：4
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