The diet of the tiger quoll (Dasyurus maculatus) in East Gippsland, Victoria, was investigated by systematically collecting seats from two latrines between December 1990 and May 1993. From the analysis of these seats, the tiger quoll was found to be a predator of vertebrate prey, largely dependent on medium-sized mammals (500 g to 5 kg). The most important prey species were the European rabbit, the common brushtail possum and the common ringtail possum. Other prey included Antechinus species, bush rats, echidnas, macropods, wombats, birds, invertebrates and reptiles. Some variation in diet occurred between seasons, due to seasonal availability of prey. A shift in diet detected between years was attributed to the variation in rainfall and the effect this had on prey species abundance. Significant differences in diet were found between adult and subadult tiger quells. Subadult quells consumed significantly more small mammals, ringtail possums, invertebrates and reptiles and significantly fewer rabbits than did adult quells. Further analysis of the tiger quells' diet, by estimating the mass contribution of prey taxa to the diet, revealed that medium-sized prey contributed more than 80% of the biomass of prey consumed.