Analysis of 338 tiger quoll scats from tableland moist forest in south-eastern New South Wales found that the greater glider was the major prey species occurring in 54.1% of scats and contributing an estimated 51.01% of the biomass consumed by tiger quolls. Medium-sized (0.5-5 kg) mammals were the most important prey group by occurrence (53.9%), frequency (66.0%) and estimated biomass contribution to diet (62.93%). Other medium-sized prey taken by tiger quolls included: long-nosed bandicoot, rabbit, brushtail possum and ringtail possum. Macropods and wombats were also present in the scats and had been presumably taken as carrion. There was no significant difference in the diets of male and female tiger quolls. Tiger quolls were recorded hunting greater gliders in their tree hollows during the day, hunting rabbits in their burrows both during the day and at night and were observed eating road-killed macropods and wombats around dawn and dusk.